Sambucus Nigra (elderberry bark) Lectin (SNA, EBL), fluorescein (FITC), is bright green SNA-fluorophore conjugate. ANS belongs to the group of type 2 ribosome-inactivating proteins and is composed of an A chain with enzymatic activity and a B chain with carbohydrate-binding activity.
ANS can induce apoptosis through the caspase-dependent pathway at low concentrations. This effect depends on the binding of B chain carbohydrates and is independent of the catalytic activity of the A chain. In colon cancers, 90% of carcinomas have been reported to express increased levels of sialic acid, linked to residues of galactose on the surface of tumor cells, which is detectable with SNA.
See the full list of fluorescent dye conjugated lectins ›
- Very bright FITC conjugate detected with traditional FITC / GFP filter set, Ex / Em: 495/515 nm
- Applications: immunofluorescence, glycobiology
- Specificity of sugar: sialic acid
- Inhibitor / eluent sugar: lactose 500 mM
- Blood group specificity: nonspecific, A> B, O
- The molecular weight of unconjugated lectin: 140 KDa
- Immunofluorescence working concentration: 5–20 µg / mL
SNA-FITC is provided at a concentration of 2 mg / ml in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. Ca2 + 0.1 mM.
A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate form during prolonged storage at 4 ° C, warm to 37 ° C and centrifuge before use.
Specification
Unit size: 2 mg
Applications
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Transfer Applications, Elispot, ELISA, Glycobiology
Recommended Use: For most applications, we recommend a freshly prepared 10 µg / ml working solution in the above buffer. A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms during prolonged storage, heat to 37 ° C and centrifuge before use.
Recommended storage: 2-8 ° C; Store froze for long-term storage
Solution: 10 mM HEPES, 015 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration: 2 mg active conjugate / ml
Conjugate: Biotinylated
Sugar specific: sialic acid