Sambucus Nigra (elderberry bark) Lectin (SNA, EBL), fluorescein (FITC), is bright green SNA-fluorophore conjugate. ANS belongs to the group of type 2 ribosome-inactivating proteins and is composed of an A chain with enzymatic activity and a B chain with carbohydrate-binding activity.
ANS can induce apoptosis through the caspase-dependent pathway at low concentrations. This effect depends on the binding of B chain carbohydrates and is independent of the catalytic activity of the A chain. In colon cancers, 90% of carcinomas have been reported to express increased levels of sialic acid, linked to residues of galactose on the surface of tumor cells, which is detectable with SNA.
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- Very bright FITC conjugate detected with traditional FITC / GFP filter set, Ex / Em: 495/515 nm
- Applications: immunofluorescence, glycobiology
- Specificity of sugar: sialic acid
- Inhibitor / eluent sugar: lactose 500 mM
- Blood group specificity: nonspecific, A> B, O
- The molecular weight of unconjugated lectin: 140 KDa
- Immunofluorescence working concentration: 5–20 µg / mL
SNA-FITC is provided at a concentration of 2 mg / ml in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. Ca2 + 0.1 mM.
A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate form during prolonged storage at 4 ° C, warm to 37 ° C and centrifuge before use.
Unit size: 2 mg
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Transfer Applications, Elispot, ELISA, Glycobiology
Recommended Use: For most applications, we recommend a freshly prepared 10 µg / ml working solution in the above buffer. A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms during prolonged storage, heat to 37 ° C and centrifuge before use.
Recommended storage: 2-8 ° C; Store froze for long-term storage
Solution: 10 mM HEPES, 015 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration: 2 mg active conjugate / ml
Sugar specific: sialic acid