Presentation

Autoantibodies to type II collagen assume an essential part in the collagen actuated joint inflammation (CIA) model. Be that as it may, autoantibodies are not consistently
fit for actuating joint pain because of the powerlessness to enact a supplement, the main basic advance in the initiation of incendiary fountains. In CIA vulnerable DBA mice, supplement fixable IgG2a and IgG2b subtype autoantibody levels, as well as their epitope explicitness, are relevant in the enlistment of joint pain. To enough concentrate on the neurotic jobs of antibodies to type II collagen, it is vital to decide individual IgG subtype autoantibody levels to mouse type II collagen as well as the heterologous sort II collagen utilized for vaccination. Then again, C57BL/6 mice, a famous strain for creating transgenic mice, produce IgG2c antibodies rather than IgG2a antibodies because of its MHC class I haplotype (H-2b). Besides, these mice can foster CIA when vaccinated with chick type II collagen. Hence, serum IgG2c autoantibody levels against mouse type II collagen and chick type II collagen ought to assessed.

C57BL/6 mice for the CIA model

Chondrex, Inc. gives Mouse Anti-Collagen IgG Antibody Subtype (IgG1, IgG2a, IgG2b, IgG2c, and IgG3) ELISA Kits for additional investigation of antibodies in mouse serum from the mouse CIA model. Contingent upon the IgG subtypes and epitopes, the immunizer antigen proclivity changes altogether among individual antibodies and serum tests from individual creatures. Accordingly, the absolute IgG fixation determined as the amount of individual IgG subtypes could not impeccably match the all out not set in stone by Chondrex, Inc’s Mouse Anti-Collagen IgG ELISA Kit. These packs can likewise be utilized to decide neutralizer levels to type I collagen (chick, cow-like, porcine, human, and mouse) upon demand. Kindly contact Chondrex, Inc. at support@chondrex.com for more data on custom kind I collagen-covered plates.

Cross-reactivity of Anti-Heterologous Type II Collagen Antibodies to Autologous Type II Collagen in Mouse CIA

This ELISA Kit is intended to decide against collagen antibodies in mouse collagen-incited joint pain model (CIA). CIA is actuated in mice, rodents and non-human primates by vaccinating with a heterolgous type II collagen as opposed to homologous collagen. In CIA-helpless mice, the serum immunizer levels to the kind II collagen utilized for inoculation are strikingly high, and significantly, these antibodies cross-respond to different types of type II collagen including autologous sort II collagen by 50-60%, because of the monitored amino corrosive arrangements of type II collagen.

Accordingly, it is critical to decide immunizer levels to a heterologous kind II collagen utilized for inoculation, however it is more vital to decide counter acting agent levels cross-responding to autologous collagen to relate neutralizer levels to improvement and seriousness of joint inflammation in CIA model. In spite of the fact that type I collagen shares 80% of its amino corrosive grouping with type II collagen, it isn’t fit for instigating immune system intervened infections, which demonstrates that B-cell and T-cell epitope explicitness is significant for laying out autoimmunity and resulting advancement of immune system illnesses. Along these lines, type I collagen may be valuable as a control for concentrating on B-cell and T-cell epitope explicitness.

Complete ELISA units to quantify mouse serum immunizer levels to different species and sorts of collagen are accessible. A standard mouse unit with one types of collagen will gauge 39 examples. Each unit contains a reference standard and reagents to perform two fractional measures. Redone units to gauge counter acting agent levels to different types of type I and type II collagen can be specially requested.

Mouse – Anti-Collagen Type I and II Antibody Assay

In CIA-helpless mice, the serum neutralizer levels to the sort II collagen utilized for vaccination are strikingly high. Because of the moderated amino corrosive successions of type II collagen, these antibodies cross-respond to autologous kind II collagen and prompt joint inflammation.

While it is essential to decide immunizer levels to the heterologous kind II collagen utilized for inoculation, it is maybe more critical to decide counter acting agent levels to autologous collagen to precisely correspond neutralizer levels with the turn of events and seriousness of joint pain in the CIA model. Chondrex, Inc. gives total ELISA units to measuring mouse IgG antibodies to different species and kinds of collagen.