Golgi-Cox impregnation1, 2 has been one of the best methods for concentrating on both the typical and strange morphology of neurons as well as glia. Utilizing the Golgi strategy, unobtrusive morphological changes in neuronal dendrites and dendritic spines have been found in the cerebrums of creatures treated with drugs as well as in the posthumous minds of patients with neurological diseases3, 4. Nonetheless, the untrustworthiness and the tedious course of Golgi staining have been significant deterrents to the far and wide utilization of this method.
FD Rapid GolgiStain Kit is planned in light of the standard of the techniques portrayed by Ramón-Moliner2, Glaser and Van der Loos5. This unit has not just drastically improved and worked on the Golgi-Cox strategy yet has additionally shown to be incredibly dependable and delicate for exhibiting morphological subtleties of neurons and glia, particularly dendritic spines. The FD Rapid GolgiStain Kit has been tried broadly and generally utilized on the cerebrums from a few types of creatures as well as on the examples of posthumous human minds.
Kit contents:
Store at room temperature
Solution A 250 ml
Solution B 250 ml
Solution C 250 ml x 2
Solution D 250 ml
Solution E 250 ml
Glass Specimen Retriever 2
Natural hair paintbrush 2
Dropping bottle 1
User Manual 1
Materials required but not included:
- Double distilled or deionized water.
- Plastic or glass tubes or vials.
- Histological supplies and equipment, including gelatin-coated microscope slides, coverslips, staining jars, ethanol, xylene or xylene substitutes, resinous mounting medium (e.g. Permount), and a light microscope.
Utilizing the Golgi strategy, unobtrusive morphological changes in neuronal dendrites and dendritic spines have been found in the cerebrums of creatures treated with drugs as well as in the after death minds of patients with neurological diseases3, 4. Notwithstanding, the untrustworthiness and the tedious course of Golgi staining have been significant impediments to the far reaching utilization of this strategy.
FD Rapid GolgiStain Kit™ (small) |
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| PK401A | FD Neurotechnologies | kit | 699.4 EUR |
FD Rapid GolgiStain™ Kit (large) |
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| PK401 | FD Neurotechnologies | kit | 881.79 EUR |
FD Rapid GolgiStain™ kit - Solution C |
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| PK401-C | FD Neurotechnologies | 250 ml | 235.2 EUR |
FD Rapid TimmStain™ Kit |
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| PK701 | FD Neurotechnologies | kit | 866.4 EUR |
FD Rapid MultiStain™ Kit |
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| PK501 | FD Neurotechnologies | kit | 721.2 EUR |
FD Apop™ Kit |
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| PK101 | FD Neurotechnologies | kit | 854.4 EUR |
FD NeuroApap™ Kit |
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| PK201 | FD Neurotechnologies | kit | 987.6 EUR |
Control Sections for FD NeuroSilver™ Kit |
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| PCS101 | FD Neurotechnologies | each | 12.02 EUR |
FD NeuroSilver™ Kit II (for 300 sections) |
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| PK301 | FD Neurotechnologies | kit | 815.11 EUR |
FD NeuroSilver™ Kit II (for 150 sections) |
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| PK301A | FD Neurotechnologies | kit | 724.63 EUR |
Control sections for FD NeuroApop™ Kit (frozen) |
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| PCS102- | FD Neurotechnologies | each | 87.6 EUR |
Control sections for FD NeuroApop™ Kit (frozen) |
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| PCS102-2 | FD Neurotechnologies | each | 80.54 EUR |
Control sections for FD NeuroApop™ Kit (paraffin) |
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| PCS102-1 | FD Neurotechnologies | each | 80.44 EUR |
FD Section Storage Solution™ |
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| PC101 | FD Neurotechnologies | 250 ml | 157.2 EUR |
FD Tissue Cryoprotection Solution™ |
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| PC102 | FD Neurotechnologies | 250 ml | 156 EUR |
FD Tissue Storage Solution™ |
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| PC103 | FD Neurotechnologies | 250 ml | 177.6 EUR |
FD Congo Red Solution™ |
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| PS108 | FD Neurotechnologies | 500 ml | 284.4 EUR |
FD Thionin Solution™ (regular strength) |
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| PS101-1 | FD Neurotechnologies | 500 ml | 206.4 EUR |
FD Thionin Solution™ (double strength) |
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| PS101-2 | FD Neurotechnologies | 500 ml | 219.6 EUR |
FD Rapid GolgiStain Kit is planned in light of the standard of the strategies portrayed by Ramón-Moliner2,
Glaser and Van der Loos5. This unit has not just drastically improved and worked on the Golgi-Cox strategy yet has additionally shown to be very dependable and delicate for exhibiting morphological subtleties of neurons and glia, particularly dendritic spines. The FD Rapid GolgiStain™ Kit has been tried broadly and generally utilized on the cerebrums from a few types of creatures as well as on the examples of posthumous human minds
References:
Corsi P. (1987) Camillo Golgi’s morphological way to deal with neuroanatomy. In Masland RL, Portera-Sanchez An and Toffano G (eds.), Neuroplasticity: another restorative apparatus in the CNS pathology, pp 1-7. Berlin: Springer.
Ramón-Moliner E. (1970) The Golgi-Cox procedure. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer.
Graveland GA, Williams RS, and DiFiglia M. (1985) Evidence for degenerative and regenerative changes in neostriatal sharp neurons in Huntington’s sickness. Science. 227:770-3. Robinson TE, and Kolb B. (1997) Persistent primary change in core accumbens and prefrontal cortex neurons delivered by past involvement in amphetamine. J. Neurosci. 17:8491-7.
Glaser ME, and Van der Loos H. (1981) Analysis of thick mind segments by front-side opposite PC microscopy: utilization of a new, high clearness Golgi-Nissl stain. J. Neurosci. Meth. 4:117-25.