Direct PCR (Tail)


Viagen’s DirectPCR® DNA Extraction System is a one-tube system for the rapid preparation of DNA from mouse tails, earpieces, yolk sacs and culture cells. Patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts from biological samples are not compatible with many molecular biology grade reactions, such as polymerase chain reaction (PCR), in part due to inhibitors contained in the crude extracts.

DirectPCR reagents not only mediate rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification while maximizing the integrity of the released genomic DNA. The simple, patent-pending procedure completely eliminates any solution transfer or tube opening steps, giving you significant additional time.

Short procedure

1. Lyse tails in DirectPCR® Lysis Reagent.
2. Incubate for 45 min at 85°C.
3. PCR genotyping with 1 µl of lysates.

Detailed protocols: tail, ear, yolk sac, and cultured cells.

The DirectPCR® system offers advantages over conventional protocols including:

  • Time-saving: Less handling time. Crude tail lysates for PCR.
  • Saving money: Profitable reactions.
  • Safe: Without organic reagents.
  • Environmental: Less waste (organic reagents, tubes, tips, etc…)
  • Reliable and Efficient: Virtually 100% success rate with high yields.

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